Biochemical and Biological Properties of Staphylococcal Enterotoxin K

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Biochemical and biological properties of Staphylococcal enterotoxin K.

Staphylococcus aureus is an important human pathogen which is implicated in a wide variety of diseases. Major determinants of the virulence of this organism include extracellular virulence factors. Staphylococcal enterotoxins (SEs) are important causative agents in staphylococcal toxic shock syndrome and food poisoning. Our study identified a novel enterotoxin, SEK, and examined its biochemical...

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Biological and immunological properties of the carboxyl terminus of staphylococcal enterotoxin C1.

Comparisons of recently published primary sequences of staphylococcal and streptococcal pyrogenic toxins prompted an evaluation of biological and immunological properties of the C terminus of staphylococcal enterotoxin C1. The 59 N-terminal amino acids were deleted from the toxin by digestion with trypsin. The resulting fragment (Mr, 20,659) contained the remaining 180 C-terminal residues. This...

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Purification and some physicochemical properties of staphylococcal enterotoxin E.

Enterotoxin E produced by Stu~hyZococcus aureus strain FRI (Food Research Institute)-326 was purified by cation exchange chromatography on carboxymethylcellulose, gel tiltration through superfine Sephadex G-75, and gel filtration in 6 M urea with superfine Sephadex G-75. The purified toxin appears to be nearly homogeneous by paper, starch gel, and polyacrylamide gel electrophoresis and double g...

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Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3.

A third staphylococcal enterotoxin C (C3) has been identified, purified, and characterized. Staphylococcal enterotoxin C3 was identified from a Staphylococcus aureus isolated received from England. The purified toxin was determined by gel permeation chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be a simple protein with a molecular weight of 26,900. The isoelect...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 2001

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.69.1.360-366.2001